Rambo - Peptide and SARM Q&A

[Pipelinesteve4]

Yes, see below.


Pentadecapeptide BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts.

Chang CH1, Tsai WC2, Hsu YH3, Pang JH4.

Abstract
BPC 157, a pentadecapeptide derived from human gastric juice, has been demonstrated to promote the healing of different tissues, including skin, muscle, bone, ligament and tendon in many animal studies. However, the underlying mechanism has not been fully clarified. The present study aimed to explore the effect of BPC 157 on tendon fibroblasts isolated from Achilles tendon of male Sprague-Dawley rat. From the result of cDNA microarray analysis, growth hormone receptor was revealed as one of the most abundantly up-regulated genes in tendon fibroblasts by BPC 157. BPC 157 dose- and time-dependently increased the expression of growth hormone receptor in tendon fibroblasts at both the mRNA and protein levels as measured by RT/real-time PCR and Western blot, respectively. The addition of growth hormone to BPC 157-treated tendon fibroblasts dose- and time-dependently increased the cell proliferation as determined by MTT assay and PCNA expression by RT/real-time PCR. Janus kinase 2, the downstream signal pathway of growth hormone receptor, was activated time-dependently by stimulating the BPC 157-treated tendon fibroblasts with growth hormone. In conclusion, the BPC 157-induced increase of growth hormone receptor in tendon fibroblasts may potentiate the proliferation-promoting effect of growth hormone and contribute to the healing of tendon.

Excellent buddy thank you I didn't have time to look for it. barbell22

 

[Pipelinesteve4]

To add, these gh secretagogues work through ghrelin to release HGH, and ghrelin also happens to increase hunger so what does that tell us? Food shouldn't be a problem.

It absolutely adds hunger. I will sleep eat all night on peptides and igf

 

[Nidnoy]

Interested in peptides to use in conjunction with test. For me an increase in hunger is a bad side I would like to avoid. It would seem as though hexarelin or one of the igf's may be good options. In your opinion which would single one would provide the most benefit between igf des, igf lr3, or hexarelin? Thanks for taking the time to answer all this.

 

[RamboStallone]

Interested in peptides to use in conjunction with test. For me an increase in hunger is a bad side I would like to avoid. It would seem as though hexarelin or one of the igf's may be good options. In your opinion which would single one would provide the most benefit between igf des, igf lr3, or hexarelin? Thanks for taking the time to answer all this.

Best bang for the buck will be Hexarelin in this situation, no doubt about it. The igfs will get expensive to run daily. They are the last "toppings" in my opinion. Test is the pie of course. GH supps are like the frosting, then you add stuff like igf. I recommend Hexarelin at 100mcgs 3x a day to start. You can add modgrf1-29 (cjc no dac) or raise the dose to 200mcgs for an extra boost. Hexarelin proved to be very effective on its own which is a big plus.

I wrote a thread on Hexarelin a couple years ago for these exact reasons. I'll dig up some of the studies out of it and share them here. Basically my favorite ghrp is Hexarelin followed by GHRP2. Ipamorelin I like prebed. One of my go to stacks is Hexarelin twice a day with Ipamorelin boom dosed prebed.

 

[Nidnoy]

Thanks for the reply. I read on another board you liked hex but wondered if thing had changed. It did not cause sleep problems or desensitization for you? I really only looking to run for about two months. Much longer than that and I get lazy doing the multiple pins daily.

I would like to do the hex twice and ipam pre bed but ipam gets expensive due to higher dose and using mod grf with it, or is that still a must? I don't have much pep experience but I did notice no difference between ghrp2 with and without mos grf. No bloods were taken just based on feel and body comp.

I took lr3 a few times. Once was great, the others were poor. The one time was everything I wanted in terms of an addition to cycle that was non aas, increase in pumps, and body comp change after adding in. The other times was like I added nothing.

What are your thoughts on the chemical synthesis vs recombinant synthesis for the igf's. does it really make a difference?

 

[RamboStallone]

Thanks for the reply. I read on another board you liked hex but wondered if thing had changed. It did not cause sleep problems or desensitization for you? I really only looking to run for about two months. Much longer than that and I get lazy doing the multiple pins daily. My methods have changed. I use MK677 as my base gh supp then cycle in and out the other peps as I desire. But when dieting or trying to avoid the hunger sides etc, I still go with Hexarelin. No sleep issues. From the studies after 16 weeks (4 months) of continuous use it was putting out half the GH output. You should be fine for 2 months.

I would like to do the hex twice and ipam pre bed but ipam gets expensive due to higher dose and using mod grf with it, or is that still a must? I don't have much pep experience but I did notice no difference between ghrp2 with and without mos grf. No bloods were taken just based on feel and body comp.I categorize them this way by best bang for the buck and overall results. 1. MK677, 2. Hexarelin or GHRP2, 3. Modgrf or CJC DAC. So no in my opinion the modgrf is not a must but if you can afford it, it will enhance the results.

I took lr3 a few times. Once was great, the others were poor. The one time was everything I wanted in terms of an addition to cycle that was non aas, increase in pumps, and body comp change after adding in. The other times was like I added nothing.

What are your thoughts on the chemical synthesis vs recombinant synthesis for the igf's. does it really make a difference?I'm not sure brother. This isn't something I ever delved into, sorry.

See bold above brother.

 

[RamboStallone]

Here's the study about desensitization I was referring to,

 

[Nidnoy]

Thanks that all makes sense. I wish mk677 did not make you so hungry. I would be a mess on that. Either I would be miserable because I was always hungry and not eating as much as I would want or I would eat a ton and that would work against my goals. My wife is Thai and a great cook, so anything that increases hunger with a lot of tempting food around is a no no for me.

 

[Nidnoy]

Any thoughts on yk11? Was all the rage for a while at pro muscle, kind of died off a bit.

 

[RamboStallone]

Thanks that all makes sense. I wish mk677 did not make you so hungry. I would be a mess on that. Either I would be miserable because I was always hungry and not eating as much as I would want or I would eat a ton and that would work against my goals. My wife is Thai and a great cook, so anything that increases hunger with a lot of tempting food around is a no no for me.

After a while the sides subside, I've been on MK677 going on 5 months and now I don't feel the hunger sides to be honest but my appetite is all over the place. What I mean is I can diet on it now, when I first used it, no way in hell could I diet on it lol! A smart way to do this would be to start MK677 during the bulk season and stay on through it when cutting since it actually preserves mass during a caloric deficit. See study below, MK677 was able to reverse diet induced catabolism (caloric deficit).


MK-677, an Orally Active Growth Hormone Secretagogue, Reverses Diet-Induced Catabolism

M. G. Murphy L. M. Plunkett B. J. Gertz W. He J. Wittreich W. M. Polvino D. R. Clemmons

The reversal of diet-induced negative nitrogen balance by GH suggests a possible therapeutic role for GH treatment in catabolic patients. A double-blind, randomized, placebo-controlled, two-period, cross-over study was designed to investigate whether MK-677, an orally active nonpeptide mimic of GH-releasing peptide, can reverse diet-induced protein catabolism. Eight healthy volunteers (ages 24–39 yr) were calorically restricted (18 kcal/kg·day) for two 14-day periods. During the last 7 days of each diet period, subjects received either oral MK-677 25 mg or placebo once daily. There was a 14- to 21-day washout interval between periods. During the first week of caloric restriction (i.e. diet alone), daily nitrogen losses were similar for both treatment groups (mean ± se; MK-677 group −2.67 ± 0.40 g/day vs. placebo group− 2.83 ± 0.26 g/day). During the second week (diet and study drug), mean daily nitrogen balance was 0.31 ± 0.21 g/day in the MK-677 treatment group compared with −1.48 ± 0.21 g/day in the placebo group (P < 0.01). MK-677 improved nitrogen balance integrated over the 7 days of treatment; area under the curve day 8–14 nitrogen balance response was +2.69 ± 5.0 (se) for MK-677 and −8.97 ± 5.26 g·day for placebo (P < 0.001). MK-677 produced a peak GH response of 55.9 ± 31.7 μg/L after single dose (day 1 of treatment) and 22.6 ± 9.3 μg/L after a week of dosing compared with placebo treatment peak GH values of approximately 9 (treatment day 1) and approximately 7 μg/L (treatment day 7). Following the initial 7-day caloric restriction, insulin-like growth factor-I (IGF-I) declined from 232 ± 25 to 186 ± 19 ng/mL in the MK-677 group and from 236 ± 19 to 174 ± 23 ng/mL in the placebo group. Mean IGF-I concentration increased significantly during MK-677 to 264 ± 31 ng/mL (mean for the last 5 days of treatment) compared with 188 ± 19 ng/mL with placebo (P < 0.01). No significant difference in IGF binding protein-2 was found between the MK-677 and placebo treatments. However, the mean in IGF binding protein-3 for the last 5 days of MK-677 treatment was also significantly increased to 3273 ± 330 ng/mL (mean ± se) compared with placebo 2604 ± 253 ng/mL (P < 0.01). Neither the serum cortisol nor the PRL response was significantly greater after 7 days of MK-677 dosing compared with 7 days of placebo. MK-677 (25 mg) was generally well tolerated and without clinically significant adverse experiences. In conclusion, MK-677 reverses diet-induced nitrogen wasting, suggesting that if these short-term anabolic effects are maintained in patients who are catabolic because of certain acute or chronic disease states, it may be useful in treating catabolic conditions.

Full Study: https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.83.2.4551

 

[RamboStallone]

Any thoughts on yk11? Was all the rage for a while at pro muscle, kind of died off a bit.

Not many have used it. I have heard from a few that it increased their strength and size significantly. It seems to be a full blown steroid chemically. Talked to a chemist briefly and looks like it is an oral nandrolone pretty much. Effects were like NPP but faster and stronger like an oral steroid. Not much more has been done with it at this point. It's still too early to tell.

 

[Nidnoy]

That sounds good but wonder about the toxicity. I searched and what little I found it doesn't appear harsh, but is new.

Would be nice to have an effective oral that didn't trash lipids and liver that was affordable. I think primo fits that in high enough doses but that price tag is too much.

 

[RamboStallone]

That sounds good but wonder about the toxicity. I searched and what little I found it doesn't appear harsh, but is new.

Would be nice to have an effective oral that didn't trash lipids and liver that was affordable. I think primo fits that in high enough doses but that price tag is too much.

It's not 17aa so liver toxicity is not an issue. However, it did give some people BP and heart rate issues so you would have to be careful and watch that. It seems to act like nandrolone from an anabolic sense but more like a DHT from an androgenic standpoint. I haven't tried it myself yet and it does peak my interest. I'd be interested in more user feedback for sure.

 

[Pipelinesteve4]

It's not 17aa so liver toxicity is not an issue. However, it did give some people BP and heart rate issues so you would have to be careful and watch that. It seems to act like nandrolone from an anabolic sense but more like a DHT from an androgenic standpoint. I haven't tried it myself yet and it does peak my interest. I'd be interested in more user feedback for sure.

User feedback is always a good thing

 

[RamboStallone]

User feedback is always a good thing

For sure, agreed. There are many times where on paper and in vitro a compound behaves one way and ends up behaving a completely different way in vivo. We will be carrying YK-11 down the line, probably a few weeks out or so.

 

[Kins]

I don't have too much personal experience with these. I'm using GW for the first time now and I am definitely noticing an endurance increase. I'm bulking so can't tell any fat loss. They are both very similar in effects, except it seems like guys who like GW, love SR. With these particular drugs, people either get the effects and love it or others notice barely anything at all. I'm waiting for my next bloodwork to see what my lipids look like. So while SR seems stronger it's drawbacks are it's more expensive and it needs to be dosed multiple times a day. Most guys preferred 3-4x a day to see real good effects.

I've gotten blood work on gw and it showed not difference in my lipid profile at all. The only thing that was significant was fasting blood glucose went down significantly. I love it for that reason only. Never saw any fat loss from it or any endurance increase.

 

[RamboStallone]

I've gotten blood work on gw and it showed not difference in my lipid profile at all. The only thing that was significant was fasting blood glucose went down significantly. I love it for that reason only. Never saw any fat loss from it or any endurance increase.

I think it lowers my bg because I do get a bit lethargic after taking it. My friend goes hypo as soon as he takes it, I've seen some others say this as well.

Thanks for sharing.

 

[Pipelinesteve4]

I think it lowers my bg because I do get a bit lethargic after taking it. My friend goes hypo as soon as he takes it, I've seen some others say this as well.

Thanks for sharing.

It gives me energy. Better than a preworkout drink. Like the old ones.

 

[Kins]

I think it lowers my bg because I do get a bit lethargic after taking it. My friend goes hypo as soon as he takes it, I've seen some others say this as well.

Thanks for sharing.

Yeah, if I don't eat enough carbs on it I come close to passing out.

 

[Nidnoy]

For sure, agreed. There are many times where on paper and in vitro a compound behaves one way and ends up behaving a completely different way in vivo. We will be carrying YK-11 down the line, probably a few weeks out or so.

That is good to hear. One of the problems with this was where to get it, either RC shops or seems like smaller supp companies, made me question legitimacy