Testing gh

[WARd1v1ng]

If one obtained hgh in powder form, how would one get it tested..id imagine it be stable in a cool/cold environment as i understand it degrades..
Ive seen the raw offered for yrs but one sponsor on here has some and i got to tjinling of if it were possible to get real raw, id split some with my buddys..so say 10iu vial is like 12mg, a kit is 120mg ,10 kits 1.2g, etc...this would be a game changer..
Or did i just mentally masturbate again?
Rambo,dred,anyone,gman...anyone have ideas..

 

[MotorCityMuscleMachine]

If one obtained hgh in powder form, how would one get it tested..id imagine it be stable in a cool/cold environment as i understand it degrades..
Ive seen the raw offered for yrs but one sponsor on here has some and i got to tjinling of if it were possible to get real raw, id split some with my buddys..so say 10iu vial is like 12mg, a kit is 120mg ,10 kits 1.2g, etc...this would be a game changer..
Or did i just mentally masturbate again?
Rambo,dred,anyone,gman...anyone have ideas..

I may be wrong, { anyone, that does know better, please correct me. }

But , GH requires very sophisticated equipment, and if you notice when you re-constitute with bac water, there is a very prominent vacumn to the vial. I don't know if I'd trust just a Raw (unspecialized packaging of "bulk" Raw if doable., Now I get what your asking, because I thought back in the day 11+ years back I thought I'd see. Similar offer's.

GH is fermented in Mouse E~coli for one, synthesized in extreme cold condition. Then vac packed for a reason.


Interesting, but don't take this little tid-bit of mine for gospel, but you just cannot go wrong with the majority that are readily available now, BlueTops, black tops , Somatropin , GreyTops , Meditropes etc , and quit available & affordable right here in good Ole' U.S. of America. to

 

[WARd1v1ng]

Yeah ive had crumbled puck no vac in vial gh yrs ago that was still good...
Im prob reaching..im gonna run peps/mk combo v4 i start back on with gh...but id like to explore this some more..
Mcm, thanks for chiming in..lets keep it rolling

 

[gesus]

spectrofluorometry and HPLC methods?

Process:

"..ChemicalsHuman growth hormone was obtained from EliLilly and Company (Indianapolis, USA). Humatropewas a gift from Eli Lilly (Istanbul, Turkey). All chemicalswere of analytical grade. Buffer solution (potassiumdihydrogen phosphate, KH2PO4) was preparedwith deionized water by using an aquaMAX™ultra, Young instrument (Korea) ultrawater purificationsystem.Spectrofluorometric and chromatographic systemAll fluorescence measurements were done on aShimadsu RF-5301 PC spectrofluorometer equippedwith a 150 W Xenon lamp. Experimental parameterswere slit width 5.0 nm, λexc = 275 nm and λem = 335 nm.The HPLC system consisted of a ThermoseparationsSpectra Series P 4000 gradient pump coupledwith a Spectra System UV 6000 detection and a ThermoseparationsAS 3000 auto sampler, a SCM 1000vacuum membrane degasser, a SN 4000 system controller.The detector was set at 220 nm, which was thewavelength used for quantification. Separation wasoperated on a Luna reversed-phase C18 column withparticle size of 5 μm (250 mm×4.6 mm i.d.) using awater mobile phase consisting of 0.1% H3PO4 at aflow rate of 0.5 ml min-1. The UV detector was operatedat 220 nm and column temperature was adjustedat 55 °C. 50 μL of samples was injected to theHPLC system.Preparation of the standard and quality controlsolutionsThe stock solution of growth hormone was preparedin 25 mM phosphate buffer solution to a concentrationof 650 μg mL-1. Phosphate buffer was preparedin double distilled filtered water and its pH wasadjusted to 7.4±0.02 with sodium hydroxide solution.Standard solutions were prepared as 1.25-50 μg mL-1(1.25, 2.5, 5, 10, 20, 30, 40 and 50 μg mL-1) forspectrofluorometry and 10-75 μg mL-1 (10, 20, 30, 40,50 and 75 μg mL-1) for the HPLC method.The quality control (QC) samples were preparedby adding aliquots of standard working solution ofgrowth hormone to final concentrations of 2, 12.5 and35 μg mL-1 for the spectrofluorometry and 15, 35 and60 μg mL-1 for the HPLC method.Procedure for pharmaceutical preparationHumatrope injectable form containing growthhormone (1.33 mg) was accurately weighed andtransferred into 10 mL volumetric flask and 2 mL 25mM phosphate buffer solution was added. Further dilutionswere performed to suit the calibration graphsfor the spectrofluorometry and HPLC methods (15and 50 μg mL-1).Data analysisAll statistical calculations were performed withthe Statistical Product and Service Solutions (SPSS)for Windows, version 10.0. Correlations were consideredstatistically significant if calculated P valueswere 0.05 or less..."

source link:

http://www.ache.org.rs/CICEQ/2012/No3/05_3374_2012_IP.pdf